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Structure paper

TitleA metal ion orients SARS-CoV-2 mRNA to ensure accurate 2'-O methylation of its first nucleotide.
Journal, issue, pagesNat Commun, Vol. 12, Page 3287-3287, Year 2021
Publish dateFeb 27, 2021 (structure data deposition date)
AuthorsViswanathan, T. / Misra, A. / Chan, S.H. / Qi, S. / Dai, N. / Arya, S. / Martinez-Sobrido, L. / Gupta, Y.K.
External linksNat Commun / PubMed:34078893
MethodsX-ray diffraction
Resolution2.3 - 2.5 Å
Structure data

PDB-7lw3:
Structure of SARS-CoV-2 nsp16/nsp10 complex in presence of Cap-1 analog (m7GpppAmU) and SAH
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7lw4:
Structure of SARS-CoV-2 nsp16/nsp10 complex in presence of S-adenosyl-L-homocysteine (SAH)
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-MG:
Unknown entry

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-YG4:
[(2~{R},3~{R},4~{R},5~{R})-5-(6-azanyl-7,8-dihydropurin-9-yl)-2-[[[[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-1,8-dihydropurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-4-methoxy-oxolan-3-yl] [(2~{R},3~{S},4~{R},5~{S})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

Source
  • severe acute respiratory syndrome coronavirus 2
KeywordsHYDROLASE / product complex

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