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TitleStructural basis for transglycosylation in glycoside hydrolase family GH116 glycosynthases.
Journal, issue, pagesArch. Biochem. Biophys., Vol. 706, Page 108924-108924, Year 2021
Publish dateNov 25, 2020 (structure data deposition date)
AuthorsPengthaisong, S. / Hua, Y. / Ketudat Cairns, J.R.
External linksArch. Biochem. Biophys. / PubMed:34019851
MethodsX-ray diffraction
Resolution1.6 - 1.95 Å
Structure data

PDB-7dks:
Crystal structure of TxGH116 E441A nucleophile mutant from Thermoanaerobacterium xylanolyticum
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-7dkt:
Crystal structure of TxGH116 E441A nucleophile mutant from Thermoanaerobacterium xylanolyticum with alpha-glucosyl fluoride
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-7dku:
Crystal structure of TxGH116 E441A nucleophile mutant from Thermoanaerobacterium xylanolyticum with cellobiose
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-7dkv:
Crystal structure of TxGH116 E441A nucleophile mutant from Thermoanaerobacterium xylanolyticum with cellotriose
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7dkw:
Crystal structure of TxGH116 E441G nucleophile mutant from Thermoanaerobacterium xylanolyticum with autocondensation products from alpha-fluoroglucoside.
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-7dkx:
Crystal structure of TxGH116 E441G nucleophile mutant from Thermoanaerobacterium xylanolyticum with cellobiose
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7dky:
Crystal structure of TxGH116 E441G nucleophile mutant from Thermoanaerobacterium xylanolyticum with cellotriose
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GLF:
alpha-D-glucopyranosyl fluoride

Source
  • thermoanaerobacterium xylanolyticum lx-11 (bacteria)
KeywordsHYDROLASE / beta-glucosidase / nucleophile mutant / cellotriose

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