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Structure paper

TitleA nanobody toolbox targeting dimeric coiled-coil modules for functionalization of designed protein origami structures.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 118, Year 2021
Publish dateAug 19, 2020 (structure data deposition date)
AuthorsMajerle, A. / Hadzi, S. / Aupic, J. / Satler, T. / Lapenta, F. / Strmsek, Z. / Lah, J. / Loris, R. / Jerala, R.
External linksProc. Natl. Acad. Sci. USA / PubMed:33893235
MethodsX-ray diffraction
Resolution1.547 - 2.694 Å
Structure data

PDB-7a48:
Crystal structure of the APH coiled-coil in complex with Nb49
Method: X-RAY DIFFRACTION / Resolution: 1.547 Å

PDB-7a4d:
Crystal structure of the APH coiled-coil in complex with nanobodies Nb28 and Nb30
Method: X-RAY DIFFRACTION / Resolution: 2.694 Å

PDB-7a4t:
Crystal structure of the GCN coiled-coil in complex with nanobody Nb39
Method: X-RAY DIFFRACTION / Resolution: 2.124 Å

PDB-7a4y:
Crystal structure of the P5P6 coiled-coil in complex with nanobody Nb34.
Method: X-RAY DIFFRACTION / Resolution: 2.157 Å

PDB-7a50:
Crystal structure of the APH coiled-coil in complex with nanobody Nb26
Method: X-RAY DIFFRACTION / Resolution: 1.999 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-GOL:
GLYCEROL

ChemComp-ACT:
ACETATE ION

ChemComp-SO4:
SULFATE ION

ChemComp-ACE:
ACETYL GROUP

Source
  • lama glama (llama)
  • synthetic construct (others)
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsDE NOVO PROTEIN / coiled-coil / nanobody / antibody / protein design

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