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TitlePOLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 118, Year 2021
Publish dateApr 30, 2020 (structure data deposition date)
AuthorsSugizaki, A. / Sato, K. / Chiba, K. / Saito, K. / Kawagishi, M. / Tomabechi, Y. / Mehta, S.B. / Ishii, H. / Sakai, N. / Shirouzu, M. ...Sugizaki, A. / Sato, K. / Chiba, K. / Saito, K. / Kawagishi, M. / Tomabechi, Y. / Mehta, S.B. / Ishii, H. / Sakai, N. / Shirouzu, M. / Tani, T. / Terada, S.
External linksProc. Natl. Acad. Sci. USA / PubMed:33674463
MethodsX-ray diffraction
Resolution2.501 Å
Structure data

PDB-7c03:
Crystal structure of POLArISact(T57S), genetically encoded probe for fluorescent polarization
Method: X-RAY DIFFRACTION / Resolution: 2.501 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • synthetic construct (others)
KeywordsLUMINESCENT PROTEIN / Fluorescent protein Fluorescent polarization Protein engineering

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