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TitleArchaeal chromatin 'slinkies' are inherently dynamic complexes with deflected DNA wrapping pathways.
Journal, issue, pagesElife, Vol. 10, Year 2021
Publish dateMar 2, 2021
AuthorsSamuel Bowerman / Jeff Wereszczynski / Karolin Luger /
PubMed AbstractEukaryotes and many archaea package their DNA with histones. While the four eukaryotic histones wrap ~147 DNA base pairs into nucleosomes, archaeal histones form 'nucleosome-like' complexes that ...Eukaryotes and many archaea package their DNA with histones. While the four eukaryotic histones wrap ~147 DNA base pairs into nucleosomes, archaeal histones form 'nucleosome-like' complexes that continuously wind between 60 and 500 base pairs of DNA ('archaeasomes'), suggested by crystal contacts and analysis of cellular chromatin. Solution structures of large archaeasomes (>90 DNA base pairs) have never been directly observed. Here, we utilize molecular dynamics simulations, analytical ultracentrifugation, and cryoEM to structurally characterize the solution state of archaeasomes on longer DNA. Simulations reveal dynamics of increased accessibility without disruption of DNA-binding or tetramerization interfaces. Mg concentration influences compaction, and cryoEM densities illustrate that DNA is wrapped in consecutive substates arranged 90 out-of-plane with one another. Without ATP-dependent remodelers, archaea may leverage these inherent dynamics to balance chromatin packing and accessibility.
External linksElife / PubMed:33650488 / PubMed Central
MethodsEM (single particle)
Resolution9.5 - 11.5 Å
Structure data

EMDB-23403:
Reconstituted 207 bp Archaeasome, Class I
Method: EM (single particle) / Resolution: 9.5 Å

EMDB-23404:
Reconsituted 207 bp Archaeasome, Class II
Method: EM (single particle) / Resolution: 11.5 Å

Source
  • Thermococcus kodakarensis (archaea)

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