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TitleInsights into the molecular determinants of thermal stability in halohydrin dehalogenase HheD2.
Journal, issue, pagesFebs J., Vol. 288, Page 4683-4701, Year 2021
Publish dateDec 9, 2020 (structure data deposition date)
AuthorsWessel, J. / Petrillo, G. / Estevez-Gay, M. / Bosch, S. / Seeger, M. / Dijkman, W.P. / Iglesias-Fernandez, J. / Hidalgo, A. / Uson, I. / Osuna, S. / Schallmey, A.
External linksFebs J. / PubMed:33605544
MethodsX-ray diffraction
Resolution1.6 Å
Structure data

PDB-7b73:
Insight into the molecular determinants of thermal stability in halohydrin dehalogenase HheD2.
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-NHE:
2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID / pH buffer*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • gamma proteobacterium htcc2207 (bacteria)
KeywordsLYASE / thermostability / enantioselectivity changes / directed evolution / protein engineering / short-chain dehydrogenase/reductase enzyme superfamily / dehalogenase

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