EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of gating and partial agonist action in the glycine receptor.
ジャーナル・号・ページ	Cell, Vol. 184, Issue 4, Page 957-968.e21, Year 2021
掲載日	2021年2月18日
著者	Jie Yu / Hongtao Zhu / Remigijus Lape / Timo Greiner / Juan Du / Wei Lü / Lucia Sivilotti / Eric Gouaux /
PubMed 要旨	Ligand-gated ion channels mediate signal transduction at chemical synapses and transition between resting, open, and desensitized states in response to neurotransmitter binding. Neurotransmitters ...Ligand-gated ion channels mediate signal transduction at chemical synapses and transition between resting, open, and desensitized states in response to neurotransmitter binding. Neurotransmitters that produce maximum open channel probabilities (Po) are full agonists, whereas those that yield lower than maximum Po are partial agonists. Cys-loop receptors are an important class of neurotransmitter receptors, yet a structure-based understanding of the mechanism of partial agonist action has proven elusive. Here, we study the glycine receptor with the full agonist glycine and the partial agonists taurine and γ-amino butyric acid (GABA). We use electrophysiology to show how partial agonists populate agonist-bound, closed channel states and cryo-EM reconstructions to illuminate the structures of intermediate, pre-open states, providing insights into previously unseen conformational states along the receptor reaction pathway. We further correlate agonist-induced conformational changes to Po across members of the receptor family, providing a hypothetical mechanism for partial and full agonist action at Cys-loop receptors.
リンク	Cell / PubMed:33567265 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 4.0 Å
構造データ	20370 6plo EMDB-20370, PDB-6plo: CryoEM structure of zebra fish alpha-1 glycine receptor YGF mutant bound with GABA in SMA,open state 手法: EM (単粒子) / 解像度: 3.3 Å 20371 6plp EMDB-20371, PDB-6plp: CryoEM structure of zebra fish alpha-1 glycine receptor YGF mutant bound with GABA in SMA, desensitized state 手法: EM (単粒子) / 解像度: 3.3 Å 20372 6plq EMDB-20372, PDB-6plq: CryoEM structure of zebra fish alpha-1 glycine receptor YGF mutant bound with GABA in SMA, super-open state 手法: EM (単粒子) / 解像度: 3.4 Å 20373 6plr EMDB-20373, PDB-6plr: CryoEM structure of zebra fish alpha-1 glycine receptor bound with glycine in nanodisc, desensitized state 手法: EM (単粒子) / 解像度: 3.2 Å 20374 6pls EMDB-20374, PDB-6pls: CryoEM structure of zebra fish alpha-1 glycine receptor bound with taurine in nanodisc, desensitized state 手法: EM (単粒子) / 解像度: 3.0 Å 20375 6plt EMDB-20375, PDB-6plt: CryoEM structure of zebra fish alpha-1 glycine receptor bound with taurine in nanodisc, closed state 手法: EM (単粒子) / 解像度: 3.2 Å 20376 6plu EMDB-20376, PDB-6plu: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in nanodisc, desensitized state 手法: EM (単粒子) / 解像度: 3.3 Å 20377 6plv EMDB-20377, PDB-6plv: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in nanodisc, closed state 手法: EM (単粒子) / 解像度: 3.3 Å 20378 6plw EMDB-20378, PDB-6plw: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, super-open state 手法: EM (単粒子) / 解像度: 3.0 Å 20379 6plx EMDB-20379, PDB-6plx: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, desensitized state 手法: EM (単粒子) / 解像度: 2.9 Å 20380 6ply EMDB-20380, PDB-6ply: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, open state 手法: EM (単粒子) / 解像度: 2.9 Å 20381 6plz EMDB-20381, PDB-6plz: CryoEM structure of zebra fish alpha-1 glycine receptor bound with GABA in SMA, closed state 手法: EM (単粒子) / 解像度: 3.0 Å 20382 6pm0 EMDB-20382, PDB-6pm0: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, super-open state 手法: EM (単粒子) / 解像度: 3.1 Å 20383 6pm1 EMDB-20383, PDB-6pm1: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, desensitized state 手法: EM (単粒子) / 解像度: 3.0 Å 20384 6pm2 EMDB-20384, PDB-6pm2: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, open state 手法: EM (単粒子) / 解像度: 3.0 Å 20385 6pm3 EMDB-20385, PDB-6pm3: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Taurine in SMA, closed state 手法: EM (単粒子) / 解像度: 3.0 Å 20386 6pm4 EMDB-20386, PDB-6pm4: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Glycine in SMA, super-open state 手法: EM (単粒子) / 解像度: 4.0 Å 20388 6pm5 EMDB-20388, PDB-6pm5: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Glycine in SMA, desensitized state 手法: EM (単粒子) / 解像度: 3.1 Å 20389 6pm6 EMDB-20389, PDB-6pm6: CryoEM structure of zebra fish alpha-1 glycine receptor bound with Glycine in SMA, open state 手法: EM (単粒子) / 解像度: 2.9 Å 20518 6pxd EMDB-20518, PDB-6pxd: CryoEM structure of zebra fish alpha-1 glycine receptor, Apo state 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-ABU: GAMMA-AMINO-BUTANOIC ACID / GABA 化合物画像なし ChemComp-UNL: Unknown ligand ChemComp-GLY: GLYCINE / グリシン ChemComp-OCT: N-OCTANE / オクタン ChemComp-TAU: 2-AMINOETHANESULFONIC ACID / タウリン ChemComp-CL: Unknown entry / クロリド ChemComp-HOH: WATER
由来	danio rerio (ゼブラフィッシュ)
キーワード	MEMBRANE PROTEIN / glycine receptor / SMA / CryoEM / nanodisc