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TitleStructure of the activated human minor spliceosome.
Journal, issue, pagesScience, Vol. 371, Issue 6535, Year 2021
Publish dateMar 19, 2021
AuthorsRui Bai / Ruixue Wan / Lin Wang / Kui Xu / Qiangfeng Zhang / Jianlin Lei / Yigong Shi /
PubMed AbstractThe minor spliceosome mediates splicing of the rare but essential U12-type precursor messenger RNA. Here, we report the atomic features of the activated human minor spliceosome determined by cryo- ...The minor spliceosome mediates splicing of the rare but essential U12-type precursor messenger RNA. Here, we report the atomic features of the activated human minor spliceosome determined by cryo-electron microscopy at 2.9-angstrom resolution. The 5' splice site and branch point sequence of the U12-type intron are recognized by the U6atac and U12 small nuclear RNAs (snRNAs), respectively. Five newly identified proteins stabilize the conformation of the catalytic center: The zinc finger protein SCNM1 functionally mimics the SF3a complex of the major spliceosome, the RBM48-ARMC7 complex binds the γ-monomethyl phosphate cap at the 5' end of U6atac snRNA, the U-box protein PPIL2 coordinates loop I of U5 snRNA and stabilizes U5 small nuclear ribonucleoprotein (snRNP), and CRIPT stabilizes U12 snRNP. Our study provides a framework for the mechanistic understanding of the function of the human minor spliceosome.
External linksScience / PubMed:33509932
MethodsEM (single particle)
Resolution2.89 - 3.24 Å
Structure data

30875

7dvq

EMDB-30875, PDB-7dvq:
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Method: EM (single particle) / Resolution: 2.89 Å

EMDB-30878:
Focused refinement map of SF3B region of the activated human minor spliceosome
Method: EM (single particle) / Resolution: 2.9 Å

EMDB-30879:
focused refinement map for the RBM48/ARMC7 region
Method: EM (single particle) / Resolution: 3.24 Å

Chemicals

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-G5J:
5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
  • Human (human)
KeywordsSPLICING / spliceosome / minor spliceosome / Bact complex / human spliceosome / PPIL2 / SCNM1 / RBM48-ARMC7 complex / U12-type intron / U6atac snRNA

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