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TitleDissecting the low catalytic capability of flavin-dependent halogenases.
Journal, issue, pagesJ. Biol. Chem., Vol. 296, Page 100068-100068, Year 2020
Publish dateAug 20, 2020 (structure data deposition date)
AuthorsPhintha, A. / Prakinee, K. / Jaruwat, A. / Lawan, N. / Visitsatthawong, S. / Kantiwiriyawanitch, C. / Songsungthong, W. / Trisrivirat, D. / Chenprakhon, P. / Mulholland, A. ...Phintha, A. / Prakinee, K. / Jaruwat, A. / Lawan, N. / Visitsatthawong, S. / Kantiwiriyawanitch, C. / Songsungthong, W. / Trisrivirat, D. / Chenprakhon, P. / Mulholland, A. / van Pee, K.H. / Chitnumsub, P. / Chaiyen, P.
External linksJ. Biol. Chem. / PubMed:33465708
MethodsX-ray diffraction
Resolution1.91 - 2.4 Å
Structure data

PDB-7cu0:
Crystal structure of Streptomyces albogriseolus flavin-dependent tryptophan 6-halogenase Thal in complex with tryptophan
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-7cu1:
CRYSTAL STRUCTURE OF STREPTOMYCES ALBOGRISEOLUS FLAVIN-DEPENDENT TRYPTOPHAN 6-HALOGENASE (THAL) IN COMPLEX WITH FAD and AMP
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

PDB-7cu2:
CRYSTAL STRUCTURE OF STREPTOMYCES ALBOGRISEOLUS FLAVIN-DEPENDENT TRYPTOPHAN 6-HALOGENASE THAL IN COMPLEX WITH REDUCED FAD
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-TRP:
TRYPTOPHAN

ChemComp-HOH:
WATER

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-FDA:
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE

Source
  • streptomyces albogriseolus (bacteria)
KeywordsOXIDOREDUCTASE / flavin monooxygenase / flavin-dependent tryptophan 6-halogenase

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