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TitleFast Kinetics Reveals Rate-Limiting Oxidation and the Role of the Aromatic Cage in the Mechanism of the Nicotine-Degrading Enzyme NicA2.
Journal, issue, pagesBiochemistry, Vol. 60, Page 259-273, Year 2021
Publish dateOct 21, 2020 (structure data deposition date)
AuthorsTararina, M.A. / Dam, K.K. / Dhingra, M. / Janda, K.D. / Palfey, B.A. / Allen, K.N.
External linksBiochemistry / PubMed:33464876
MethodsX-ray diffraction
Resolution2.31 - 2.69 Å
Structure data

PDB-7khn:
NicA2 variant N462Y/W427Y in complex with (S)-nicotine
Method: X-RAY DIFFRACTION / Resolution: 2.31 Å

PDB-7kho:
NicA2 variant N462V in complex with (S)-nicotine
Method: X-RAY DIFFRACTION / Resolution: 2.69 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-NCT:
(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE / alkaloid*YM

ChemComp-HOH:
WATER

Source
  • pseudomonas putida (strain s16) (bacteria)
  • pseudomonas putida s16 (bacteria)
KeywordsFLAVOPROTEIN / flavoenzyme / oxidoreductase / nicotine / Rossmann-core fold

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