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Structure paper

TitleThe Card1 nuclease provides defence during type III CRISPR immunity.
Journal, issue, pagesNature, Vol. 590, Page 624-629, Year 2021
Publish dateMay 12, 2020 (structure data deposition date)
AuthorsRostol, J.T. / Xie, W. / Kuryavyi, V. / Maguin, P. / Kao, K. / Froom, R. / Patel, D.J. / Marraffini, L.A.
External linksNature / PubMed:33461211
MethodsX-ray diffraction
Resolution1.95 - 3 Å
Structure data

PDB-6wxw:
crystal structure of apo Card1
Method: X-RAY DIFFRACTION / Resolution: 2.32 Å

PDB-6wxx:
crystal structure of cA4-activated Card1
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-6wxy:
crystal structure of cA6-bound Card1
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6xl1:
crystal structure of cA4-activated Card1(D294N)
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

Source
  • treponema succinifaciens (strain atcc 33096 / dsm 2489 / 6091) (bacteria)
  • synthetic construct (others)
  • treponema succinifaciens dsm 2489 (bacteria)
KeywordsDNA BINDING PROTEIN / Card1 / Dnase / cyclic oligoadenylates / DNA BINDING PROTEIN/RNA / DNA BINDING PROTEIN-RNA complex / Cad1

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