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Structure paper

TitleMolecular underpinnings of ssDNA specificity by Rep HUH-endonucleases and implications for HUH-tag multiplexing and engineering.
Journal, issue, pagesNucleic Acids Res., Vol. 49, Page 1046-1064, Year 2021
Publish dateApr 1, 2020 (structure data deposition date)
AuthorsTompkins, K.J. / Houtti, M. / Litzau, L.A. / Aird, E.J. / Everett, B.A. / Nelson, A.T. / Pornschloegl, L. / Limon-Swanson, L.K. / Evans, R.L. / Evans, K. ...Tompkins, K.J. / Houtti, M. / Litzau, L.A. / Aird, E.J. / Everett, B.A. / Nelson, A.T. / Pornschloegl, L. / Limon-Swanson, L.K. / Evans, R.L. / Evans, K. / Shi, K. / Aihara, H. / Gordon, W.R.
External linksNucleic Acids Res. / PubMed:33410911
MethodsX-ray diffraction
Resolution1.8 - 2.612 Å
Structure data

PDB-6wdz:
Porcine circovirus 2 Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site
Method: X-RAY DIFFRACTION / Resolution: 2.03 Å

PDB-6we0:
Wheat dwarf virus Rep domain complexed with a single-stranded DNA 10-mer comprising the cleavage site
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6we1:
Wheat dwarf virus Rep domain complexed with a single-stranded DNA 8-mer comprising the cleavage site
Method: X-RAY DIFFRACTION / Resolution: 2.612 Å

Chemicals

ChemComp-MN:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • porcine circovirus 2
  • wheat dwarf virus
KeywordsREPLICATION/DNA / HUH-tag / HUH motif / Rep domain / viral protein / single stranded DNA / ssDNA / ssDNA binding / REPLICATION / DNA BINDING PROTEIN / REPLICATION-DNA complex / replicase

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