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TitleDirected Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning.
Journal, issue, pagesCell, Vol. 183, Page 1986-2002.e26, Year 2020
Publish dateJun 20, 2019 (structure data deposition date)
AuthorsUnger, E.K. / Keller, J.P. / Altermatt, M. / Liang, R. / Matsui, A. / Dong, C. / Hon, O.J. / Yao, Z. / Sun, J. / Banala, S. ...Unger, E.K. / Keller, J.P. / Altermatt, M. / Liang, R. / Matsui, A. / Dong, C. / Hon, O.J. / Yao, Z. / Sun, J. / Banala, S. / Flanigan, M.E. / Jaffe, D.A. / Hartanto, S. / Carlen, J. / Mizuno, G.O. / Borden, P.M. / Shivange, A.V. / Cameron, L.P. / Sinning, S. / Underhill, S.M. / Olson, D.E. / Amara, S.G. / Temple Lang, D. / Rudnick, G. / Marvin, J.S. / Lavis, L.D. / Lester, H.A. / Alvarez, V.A. / Fisher, A.J. / Prescher, J.A. / Kash, T.L. / Yarov-Yarovoy, V. / Gradinaru, V. / Looger, L.L. / Tian, L.
External linksCell / PubMed:33333022
MethodsX-ray diffraction
Resolution2.1 Å
Structure data

PDB-6per:
Crystal Structure of Ligand-Free iSeroSnFR
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

Source
  • thermoanaerobacter sp. x513 (bacteria)
KeywordsFLUORESCENT PROTEIN / fluorescent sensor / serotonin binding protein / biosensor

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