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Structure paper

TitleAllosteric cooperation in a de novo-designed two-domain protein.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 117, Page 33246-33253, Year 2020
Publish dateJul 20, 2020 (structure data deposition date)
AuthorsPirro, F. / Schmidt, N. / Lincoff, J. / Widel, Z.X. / Polizzi, N.F. / Liu, L. / Therien, M.J. / Grabe, M. / Chino, M. / Lombardi, A. / DeGrado, W.F.
External linksProc. Natl. Acad. Sci. USA / PubMed:33318174
MethodsX-ray diffraction
Resolution3.5 Å
Structure data

PDB-7jh6:
De novo designed two-domain di-Zn(II) and porphyrin-binding protein
Method: X-RAY DIFFRACTION / Resolution: 3.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-7BU:
[5,10,15,20-tetrakis(trifluoromethyl)porphyrinato(2-)-kappa~4~N~21~,N~22~,N~23~,N~24~]zinc

ChemComp-2PE:
NONAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

Source
  • synthetic construct (others)
KeywordsDE NOVO PROTEIN / Metalloprotein / inter-domain coupling and cooperation / de novo protein design / allostery / synthetic porphyrin

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