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Title | Diverse CRISPR-Cas Complexes Require Independent Translation of Small and Large Subunits from a Single Gene. |
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Journal, issue, pages | Mol Cell, Vol. 80, Issue 6, Page 971-979.e7, Year 2020 |
Publish date | Dec 17, 2020 |
Authors | Tess M McBride / Evan A Schwartz / Abhishek Kumar / David W Taylor / Peter C Fineran / Robert D Fagerlund / |
PubMed Abstract | CRISPR-Cas adaptive immune systems provide prokaryotes with defense against viruses by degradation of specific invading nucleic acids. Despite advances in the biotechnological exploitation of select ...CRISPR-Cas adaptive immune systems provide prokaryotes with defense against viruses by degradation of specific invading nucleic acids. Despite advances in the biotechnological exploitation of select systems, multiple CRISPR-Cas types remain uncharacterized. Here, we investigated the previously uncharacterized type I-D interference complex and revealed that it is a genetic and structural hybrid with similarity to both type I and type III systems. Surprisingly, formation of the functional complex required internal in-frame translation of small subunits from within the large subunit gene. We further show that internal translation to generate small subunits is widespread across diverse type I-D, I-B, and I-C systems, which account for roughly one quarter of CRISPR-Cas systems. Our work reveals the unexpected expansion of protein coding potential from within single cas genes, which has important implications for understanding CRISPR-Cas function and evolution. |
External links | Mol Cell / PubMed:33248026 |
Methods | EM (single particle) |
Resolution | 7.2 - 19.0 Å |
Structure data | EMDB-21607: EMDB-22912: |
Source |
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