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Structure paper

TitleThe RNA Polymerase alpha Subunit Recognizes the DNA Shape of the Upstream Promoter Element.
Journal, issue, pagesBiochemistry, Vol. 59, Page 4523-4532, Year 2020
Publish dateMay 21, 2010 (structure data deposition date)
AuthorsLara-Gonzalez, S. / Dantas Machado, A.C. / Rao, S. / Napoli, A.A. / Birktoft, J. / Di Felice, R. / Rohs, R. / Lawson, C.L.
External linksBiochemistry / PubMed:33205945
MethodsX-ray diffraction
Resolution2.987 - 3.252 Å
Structure data

PDB-3n4m:
E. coli RNA polymerase alpha subunit C-terminal domain in complex with CAP and DNA
Method: X-RAY DIFFRACTION / Resolution: 2.987 Å

PDB-3n97:
RNA polymerase alpha C-terminal domain (E. coli) and sigma region 4 (T. aq. mutant) bound to (UP,-35 element) DNA
Method: X-RAY DIFFRACTION / Resolution: 3.252 Å

Chemicals

ChemComp-CMP:
ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

Source
  • escherichia coli (E. coli)
  • thermus aquaticus (bacteria)
KeywordsGENE REGULATION/DNA / protein-protein interactions / protein-DNA interactions / GENE REGULATION-DNA complex / transcription initiation

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