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Structure paper

TitleStructure and reconstitution of a hydrolase complex that may release peptidoglycan from the membrane after polymerization.
Journal, issue, pagesNat Microbiol, Vol. 6, Page 34-43, Year 2021
Publish dateAug 14, 2019 (structure data deposition date)
AuthorsSchaefer, K. / Owens, T.W. / Page, J.E. / Santiago, M. / Kahne, D. / Walker, S.
External linksNat Microbiol / PubMed:33168989
MethodsX-ray diffraction
Resolution2.6 Å
Structure data

PDB-6u0o:
Crystal structure of a peptidoglycan release complex, SagB-SpdC, in lipidic cubic phase
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

Chemicals

ChemComp-FLC:
CITRATE ANION

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-SO4:
SULFATE ION

ChemComp-AE3:
2-(2-ETHOXYETHOXY)ETHANOL

ChemComp-HOH:
WATER

Source
  • staphylococcus aureus (strain bovine rf122 / et3-1) (bacteria)
  • staphylococcus aureus (strain nctc 8325) (bacteria)
  • synthetic construct (others)
KeywordsHYDROLASE / Glucosaminidase / Peptidoglycan hydrolase / CAAX protease

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