Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of inhibitor-bound mammalian complex I.
Journal, issue, pages	Nat Commun, Vol. 11, Issue 1, Page 5261, Year 2020
Publish date	Oct 16, 2020
Authors	Hannah R Bridges / Justin G Fedor / James N Blaza / Andrea Di Luca / Alexander Jussupow / Owen D Jarman / John J Wright / Ahmed-Noor A Agip / Ana P Gamiz-Hernandez / Maxie M Roessler / Ville R I Kaila / Judy Hirst /
PubMed Abstract	Respiratory complex I (NADH:ubiquinone oxidoreductase) captures the free energy from oxidising NADH and reducing ubiquinone to drive protons across the mitochondrial inner membrane and power ...Respiratory complex I (NADH:ubiquinone oxidoreductase) captures the free energy from oxidising NADH and reducing ubiquinone to drive protons across the mitochondrial inner membrane and power oxidative phosphorylation. Recent cryo-EM analyses have produced near-complete models of the mammalian complex, but leave the molecular principles of its long-range energy coupling mechanism open to debate. Here, we describe the 3.0-Å resolution cryo-EM structure of complex I from mouse heart mitochondria with a substrate-like inhibitor, piericidin A, bound in the ubiquinone-binding active site. We combine our structural analyses with both functional and computational studies to demonstrate competitive inhibitor binding poses and provide evidence that two inhibitor molecules bind end-to-end in the long substrate binding channel. Our findings reveal information about the mechanisms of inhibition and substrate reduction that are central for understanding the principles of energy transduction in mammalian complex I.
External links	Nat Commun / PubMed:33067417 / PubMed Central
Methods	EM (single particle)
Resolution	3.0 - 3.1 Å
Structure data	11377 6zr2 6ztq EMDB-11377, PDB-6zr2: Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A PDB-6ztq: Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A Method: EM (single particle) / Resolution: 3.1 Å 11424 6zr2 6ztq EMDB-11424, PDB-6ztq: Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A PDB-6zr2: Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Method: EM (single particle) / Resolution: 3.0 Å 11425 6zr2 EMDB-11425: Cryo-EM structure of respiratory complex I from Mus musculus inhibited by piericidin A at 3.0 A (Falcon 3) PDB-6zr2: Cryo-EM structure of respiratory complex I in the active state from Mus musculus at 3.1 A Method: EM (single particle) / Resolution: 3.0 Å
Chemicals	ChemComp-SF4: IRON/SULFUR CLUSTER / Iron–sulfur cluster ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipidYM / Phosphatidylcholine ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster ChemComp-FMN: FLAVIN MONONUCLEOTIDE / Flavin mononucleotide ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipidYM / Phosphatidylethanolamine ChemComp-CDL: CARDIOLIPIN / phospholipidYM / Cardiolipin ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM / Adenosine triphosphate ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate ChemComp-ZN: Unknown entry ChemComp-EHZ: ~{S}-[2-[3-[[(2~{R})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{S})-3-oxidanyltetradecanethioate ChemComp-HQH: Piericidin A / antibiotic*YM / Piericidin A
Source	mus musculus (house mouse) Mouse (mice)
Keywords	OXIDOREDUCTASE / NADH / ubiquinone / complex I