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TitleSelective inhibition of human translation termination by a drug-like compound.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 4941, Year 2020
Publish dateOct 2, 2020
AuthorsWenfei Li / Stacey Tsai-Lan Chang / Fred R Ward / Jamie H D Cate /
PubMed AbstractMethods to directly inhibit gene expression using small molecules hold promise for the development of new therapeutics targeting proteins that have evaded previous attempts at drug discovery. Among ...Methods to directly inhibit gene expression using small molecules hold promise for the development of new therapeutics targeting proteins that have evaded previous attempts at drug discovery. Among these, small molecules including the drug-like compound PF-06446846 (PF846) selectively inhibit the synthesis of specific proteins, by stalling translation elongation. These molecules also inhibit translation termination by an unknown mechanism. Using cryo-electron microscopy (cryo-EM) and biochemical approaches, we show that PF846 inhibits translation termination by arresting the nascent chain (NC) in the ribosome exit tunnel. The arrested NC adopts a compact α-helical conformation that induces 28 S rRNA nucleotide rearrangements that suppress the peptidyl transferase center (PTC) catalytic activity stimulated by eukaryotic release factor 1 (eRF1). These data support a mechanism of action for a small molecule targeting translation that suppresses peptidyl-tRNA hydrolysis promoted by eRF1, revealing principles of eukaryotic translation termination and laying the foundation for new therapeutic strategies.
External linksNat Commun / PubMed:33009412 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 2.9 Å
Structure data

22085

6xa1

EMDB-22085: Selectively stalling of translation termination by a drug-like compound
PDB-6xa1: Structure of a drug-like compound stalled human translation termination complex
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-22086:
Intermediate ribosome nascent chain complex during PF846 stalled translation termination
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-MVM:
N-(3-chloropyridin-2-yl)-N-[(3R)-piperidin-3-yl]-4-(3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)benzamide

ChemComp-ZN:
Unknown entry

ChemComp-K:
Unknown entry

Source
  • homo sapiens (human)
KeywordsRIBOSOME / Selectively stalling / translation termination / drug-like compound / human ribosome

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