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Structure paper

TitleAnaerobic fixed-target serial crystallography.
Journal, issue, pagesIucrj, Vol. 7, Page 901-912, Year 2020
Publish dateFeb 10, 2020 (structure data deposition date)
AuthorsRabe, P. / Beale, J.H. / Butryn, A. / Aller, P. / Dirr, A. / Lang, P.A. / Axford, D.N. / Carr, S.B. / Leissing, T.M. / McDonough, M.A. ...Rabe, P. / Beale, J.H. / Butryn, A. / Aller, P. / Dirr, A. / Lang, P.A. / Axford, D.N. / Carr, S.B. / Leissing, T.M. / McDonough, M.A. / Davy, B. / Ebrahim, A. / Orlans, J. / Storm, S.L.S. / Orville, A.M. / Schofield, C.J. / Owen, R.L.
External linksIucrj / PubMed:32939282
MethodsX-ray diffraction
Resolution1.7 - 2.2 Å
Structure data

PDB-6y0n:
Arginine hydroxylase VioC in complex with Arg, 2OG and Fe under anaerobic environment using FT-SSX methods
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

PDB-6y0o:
isopenicillin N synthase in complex with ACV and Fe under anaerobic environment using FT-SSX methods
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6y0q:
Alpha-ketoglutarate-dependent dioxygenase AlkB in complex with Fe, AKG and methylated DNA under anaerobic environment using FT-SSX methods
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6y12:
Arginine hydroxylase VioC in complex with (3S)-OH-Arg, succinate and Fe after oxygen exposure using FT-SSX methods
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-6ypv:
Alpha-ketoglutarate-dependent dioxygenase AlkB in complex with Fe and AKG after oxygen exposure using FT-SSX methods
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-ARG:
ARGININE

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-FE:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-SO4:
SULFATE ION

ChemComp-ACV:
L-D-(A-AMINOADIPOYL)-L-CYSTEINYL-D-VALINE

ChemComp-O5W:
[(3~{S},5~{R})-5-(6-azanyl-1-methyl-purin-9-yl)-2-(phosphonooxymethyl)oxolan-3-yl] [(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methyl hydrogen phosphate

ChemComp-SIN:
SUCCINIC ACID

ChemComp-ZZU:
(2S,3S)-3-HYDROXYARGININE

Source
  • streptomyces vinaceus (bacteria)
  • aspergillus nidulans fgsc a4 (mold)
  • escherichia coli k-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
KeywordsOXIDOREDUCTASE / iron dependent oxygenase / penicillin biosynthesis / isopenicillin N / iron dependant dioxygenase / DNA repair / 2-oxoglutarate / Arginine hydroxylase / iron dependent dioxygenase / DNA repait

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