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-Structure paper
タイトル | The flagellar motor of undergoes major structural remodeling during rotational switching. |
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ジャーナル・号・ページ | Elife, Vol. 9, Year 2020 |
掲載日 | 2020年9月7日 |
著者 | Brittany L Carroll / Tatsuro Nishikino / Wangbiao Guo / Shiwei Zhu / Seiji Kojima / Michio Homma / Jun Liu / |
PubMed 要旨 | The bacterial flagellar motor switches rotational direction between counterclockwise (CCW) and clockwise (CW) to direct the migration of the cell. The cytoplasmic ring (C-ring) of the motor, which is ...The bacterial flagellar motor switches rotational direction between counterclockwise (CCW) and clockwise (CW) to direct the migration of the cell. The cytoplasmic ring (C-ring) of the motor, which is composed of FliG, FliM, and FliN, is known for controlling the rotational sense of the flagellum. However, the mechanism underlying rotational switching remains elusive. Here, we deployed cryo-electron tomography to visualize the C-ring in two rotational biased mutants in . We determined the C-ring molecular architectures, providing novel insights into the mechanism of rotational switching. We report that the C-ring maintained 34-fold symmetry in both rotational senses, and the protein composition remained constant. The two structures show FliG conformational changes elicit a large conformational rearrangement of the rotor complex that coincides with rotational switching of the flagellum. FliM and FliN form a stable spiral-shaped base of the C-ring, likely stabilizing the C-ring during the conformational remodeling. |
リンク | Elife / PubMed:32893817 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-21819: EMDB-21837: |
由来 |
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