EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of SERCA2b reveal the mechanism of regulation by the luminal extension tail.
ジャーナル・号・ページ	Sci Adv, Vol. 6, Issue 33, Page eabb0147, Year 2020
掲載日	2020年8月12日
著者	Yuxia Zhang / Michio Inoue / Akihisa Tsutsumi / Satoshi Watanabe / Tomohiro Nishizawa / Kazuhiro Nagata / Masahide Kikkawa / Kenji Inaba /
PubMed 要旨	Sarco/endoplasmic reticulum Ca ATPase (SERCA) pumps Ca from the cytosol into the ER and maintains the cellular calcium homeostasis. Herein, we present cryo-electron microscopy (cryo-EM) structures of ...Sarco/endoplasmic reticulum Ca ATPase (SERCA) pumps Ca from the cytosol into the ER and maintains the cellular calcium homeostasis. Herein, we present cryo-electron microscopy (cryo-EM) structures of human SERCA2b in E1∙2Ca-adenylyl methylenediphosphonate (AMPPCP) and E2-BeF states at 2.9- and 2.8-Å resolutions, respectively. The structures revealed that the luminal extension tail (LE) characteristic of SERCA2b runs parallel to the lipid-water boundary near the luminal ends of transmembrane (TM) helices TM10 and TM7 and approaches the luminal loop flanked by TM7 and TM8. While the LE served to stabilize the cytosolic and TM domain arrangement of SERCA2b, deletion of the LE rendered the overall conformation resemble that of SERCA1a and SERCA2a and allowed multiple conformations. Thus, the LE appears to play a critical role in conformational regulation in SERCA2b, which likely explains the different kinetic properties of SERCA2b from those of other isoforms lacking the LE.
リンク	Sci Adv / PubMed:32851169 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.4 Å
構造データ	0912 6lle EMDB-0912: WT transporter state2 PDB-6lle: CryoEM structure of SERCA2b WT in E1-2Ca2+-AMPPCP state. 手法: EM (単粒子) / 解像度: 2.9 Å 0915 6lly EMDB-0915: WT transporter state1 PDB-6lly: CryoEM structure of SERCA2b WT in E2-BeF3- state 手法: EM (単粒子) / 解像度: 2.8 Å 0924 6ln5 EMDB-0924, PDB-6ln5: CryoEM structure of SERCA2b T1032stop in E1-2Ca2+-AMPPCP (class1) 手法: EM (単粒子) / 解像度: 2.8 Å 0925 6ln6 EMDB-0925: mutation transporter state2-2 PDB-6ln6: CryoEM structure of SERCA2b T1032stop in E1-2Ca2+-AMPPCP (class2) 手法: EM (単粒子) / 解像度: 2.9 Å 0926 6ln7 EMDB-0926: mutation transporter state2-3 PDB-6ln7: CryoEM structure of SERCA2b T1032stop in E1-2Ca2+-AMPPCP (class3) 手法: EM (単粒子) / 解像度: 2.8 Å 0927 6ln8 EMDB-0927: mutation transporter state1-1 PDB-6ln8: CryoEM structure of SERCA2b T1032stop in E2-BeF3- state (class1) 手法: EM (単粒子) / 解像度: 3.1 Å 0928 6ln9 EMDB-0928, PDB-6ln9: CryoEM structure of SERCA2b T1032stop in E2-BeF3- state (class2) 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-ACP: PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / β,γ-メチレンATP / AMP-PCP, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-BEF*: BERYLLIUM TRIFLUORIDE ION
由来	homo sapiens (ヒト)
キーワード	METAL TRANSPORT / calcium / cacium