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Structure paper

TitleSarecycline interferes with tRNA accommodation and tethers mRNA to the 70S ribosome.
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Vol. 117, Page 20530-20537, Year 2020
Publish dateJul 9, 2020 (structure data deposition date)
AuthorsBatool, Z. / Lomakin, I.B. / Polikanov, Y.S. / Bunick, C.G.
External linksProc. Natl. Acad. Sci. USA / PubMed:32817463
MethodsX-ray diffraction
Resolution2.8 - 3 Å
Structure data

PDB-6xqd:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UUC-mRNA, and deacylated P-site tRNA at 2.80A resolution
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6xqe:
Crystal structure of the Thermus thermophilus 70S ribosome in complex with sarecycline, UAA-mRNA, and deacylated P-site tRNA at 3.00A resolution
Method: X-RAY DIFFRACTION / Resolution: 3 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-V7A:
Sarecycline / medication, antibiotic*YM

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-HOH:
WATER

ChemComp-K:
Unknown entry

Source
  • escherichia coli (E. coli)
  • thermus thermophilus hb8 (bacteria)
  • escherichia virus t4
KeywordsRIBOSOME / Sarecycline; tetracycline; antibiotic; 70S ribosome; X-ray structure; inhibition of translation; decoding center; acne vulgaris

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