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TitleArtificial Intracellular Filaments.
Journal, issue, pagesCell Rep Phys Sci, Vol. 1, Issue 7, Year 2020
Publish dateJul 22, 2020
AuthorsZhaoqianqi Feng / Huaimin Wang / Fengbin Wang / Younghoon Oh / Cristina Berciu / Qiang Cui / Edward H Egelman / Bing Xu /
PubMed AbstractIntracellular protein filaments are ubiquitous for cellular functions, but forming bona fide biomimetic intracellular filaments of small molecules in living cells remains elusive. Here, we report the ...Intracellular protein filaments are ubiquitous for cellular functions, but forming bona fide biomimetic intracellular filaments of small molecules in living cells remains elusive. Here, we report the formation of self-limiting intracellular filaments of a small peptide via enzymatic morphological transition of a phosphorylated and trimethylated heterochiral tetrapeptide. Enzymatic dephosphorylation reduces repulsive intermolecular electrostatic interactions and converts the peptidic nanoparticles into filaments, which exhibit distinct types of cross-β structures with either C7 or C2 symmetries, with the hydrophilic C-terminal residues at the periphery of the helix. Macromolecular crowding promotes the peptide filaments to form bundles, which extend from the plasma membrane to nuclear membrane and hardly interact with endogenous components, including cytoskeletons. Stereochemistry and post-translational modification (PTM) of peptides are critical for generating the intracellular bundles. This work may offer a way to gain lost functions or to provide molecular insights for understanding normal and aberrant intracellular filaments.
External linksCell Rep Phys Sci / PubMed:32776017 / PubMed Central
MethodsEM (helical sym.)
Resolution4.3 Å
Structure data

22051

6x5i

EMDB-22051, PDB-6x5i:
Cryo-EM of peptide-like filament of 1-KMe3
Method: EM (helical sym.) / Resolution: 4.3 Å

Source
  • homo sapiens (human)
KeywordsPROTEIN FIBRIL / peptide-like fibril / helical symmetry

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