[English] 日本語
Yorodumi Papers
- Database of articles cited by EMDB/PDB/SASBDB data -

+
Search query

Keywords
Structure methods
Author
Journal
IF

-
Structure paper

TitleA self-supervised workflow for particle picking in cryo-EM.
Journal, issue, pagesIUCrJ, Vol. 7, Issue Pt 4, Page 719-727, Year 2020
Publish dateJul 1, 2020
AuthorsDonal M McSweeney / Sean M McSweeney / Qun Liu /
PubMed AbstractHigh-resolution single-particle cryo-EM data analysis relies on accurate particle picking. To facilitate the particle picking process, a self-supervised workflow has been developed. This includes an ...High-resolution single-particle cryo-EM data analysis relies on accurate particle picking. To facilitate the particle picking process, a self-supervised workflow has been developed. This includes an iterative strategy, which uses a 2D class average to improve training particles, and a progressively improved convolutional neural network for particle picking. To automate the selection of particles, a threshold is defined (%/Res) using the ratio of percentage class distribution and resolution as a cutoff. This workflow has been tested using six publicly available data sets with different particle sizes and shapes, and can automatically pick particles with minimal user input. The picked particles support high-resolution reconstructions at 3.0 Å or better. This workflow is a step towards automated single-particle cryo-EM data analysis at the stage of particle picking. It may be used in conjunction with commonly used single-particle analysis packages such as , , , and .
External linksIUCrJ / PubMed:32695418 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 3.0 Å
Structure data

EMDB-22025:
Reprocessing of EMPIAR-10204 data (beta-galactosidase) with particles picked by Kpicker
Method: EM (single particle) / Resolution: 2.66 Å

EMDB-22026:
Reprocessing of EMPIAR-10218 data (20S proteasome) with particles picked by Kpicker
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-22027:
Reprocessing of EMPIAR-10184 data (aldolase) with particles picked by Kpicker
Method: EM (single particle) / Resolution: 2.45 Å

EMDB-22028:
Reprocessing of EMPIAR-10059 data (TRPV1 in complex with DkTx and RTX) with particles picked by Kpicker
Method: EM (single particle) / Resolution: 3.0 Å

EMDB-22029:
Reprocessing of EMPIAR-10028 data (80S ribosome) with particles picked by Kpicker
Method: EM (single particle) / Resolution: 2.85 Å

EMDB-22030:
Reprocessing of EMPIAR-10335 data (streptavidin) with particles picked by Kpicker
Method: EM (single particle) / Resolution: 2.69 Å

Source
  • Escherichia coli (E. coli)
  • Thermoplasma acidophilum (acidophilic)
  • Oryctolagus cuniculus (rabbit)
  • Rattus norvegicus (Norway rat)
  • Plasmodium falciparum (malaria parasite P. falciparum)
  • Streptomyces avidinii (bacteria)

+
About Yorodumi Papers

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi Papers

Database of articles cited by EMDB/PDB/SASBDB data

  • Database of articles cited by EMDB, PDB, and SASBDB entries
  • Using PubMed data

Related info.:EMDB / PDB / SASBDB / Yorodumi / EMN Papers / Changes in new EM Navigator and Yorodumi

Read more