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TitleCryo-EM analysis of a membrane protein embedded in the liposome.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 117, Issue 31, Page 18497-18503, Year 2020
Publish dateAug 4, 2020
AuthorsXia Yao / Xiao Fan / Nieng Yan /
PubMed AbstractMembrane proteins (MPs) used to be the most difficult targets for structural biology when X-ray crystallography was the mainstream approach. With the resolution revolution of single-particle electron ...Membrane proteins (MPs) used to be the most difficult targets for structural biology when X-ray crystallography was the mainstream approach. With the resolution revolution of single-particle electron cryo-microscopy (cryo-EM), rapid progress has been made for structural elucidation of isolated MPs. The next challenge is to preserve the electrochemical gradients and membrane curvature for a comprehensive structural elucidation of MPs that rely on these chemical and physical properties for their biological functions. Toward this goal, here we present a convenient workflow for cryo-EM structural analysis of MPs embedded in liposomes, using the well-characterized AcrB as a prototype. Combining optimized proteoliposome isolation, cryo-sample preparation on graphene grids, and an efficient particle selection strategy, the three-dimensional (3D) reconstruction of AcrB embedded in liposomes was obtained at 3.9 Å resolution. The conformation of the homotrimeric AcrB remains the same when the surrounding membranes display different curvatures. Our approach, which can be widely applied to cryo-EM analysis of MPs with distinctive soluble domains, lays out the foundation for cryo-EM analysis of integral or peripheral MPs whose functions are affected by transmembrane electrochemical gradients or/and membrane curvatures.
External linksProc Natl Acad Sci U S A / PubMed:32680969 / PubMed Central
MethodsEM (single particle)
Resolution3.9 Å
Structure data

EMDB-22050:
3.9 Angstrom reconstruction of E.coli AcrB embedded in the liposome
Method: EM (single particle) / Resolution: 3.9 Å

Source
  • Escherichia coli (E. coli)

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