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TitleStructural characterization of a novel human adeno-associated virus capsid with neurotropic properties.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 3279, Year 2020
Publish dateJun 30, 2020
AuthorsHung-Lun Hsu / Alexander Brown / Anna B Loveland / Anoushka Lotun / Meiyu Xu / Li Luo / Guangchao Xu / Jia Li / Lingzhi Ren / Qin Su / Dominic J Gessler / Yuquan Wei / Phillip W L Tai / Andrei A Korostelev / Guangping Gao /
PubMed AbstractRecombinant adeno-associated viruses (rAAVs) are currently considered the safest and most reliable gene delivery vehicles for human gene therapy. Three serotype capsids, AAV1, AAV2, and AAV9, have ...Recombinant adeno-associated viruses (rAAVs) are currently considered the safest and most reliable gene delivery vehicles for human gene therapy. Three serotype capsids, AAV1, AAV2, and AAV9, have been approved for commercial use in patients, but they may not be suitable for all therapeutic contexts. Here, we describe a novel capsid identified in a human clinical sample by high-throughput, long-read sequencing. The capsid, which we have named AAVv66, shares high sequence similarity with AAV2. We demonstrate that compared to AAV2, AAVv66 exhibits enhanced production yields, virion stability, and CNS transduction. Unique structural properties of AAVv66 visualized by cryo-EM at 2.5-Å resolution, suggest that critical residues at the three-fold protrusion and at the interface of the five-fold axis of symmetry likely contribute to the beneficial characteristics of AAVv66. Our findings underscore the potential of AAVv66 as a gene therapy vector.
External linksNat Commun / PubMed:32606306 / PubMed Central
MethodsEM (single particle)
Resolution2.46 Å
Structure data

20630

6u3q

EMDB-20630, PDB-6u3q:
The atomic structure of a human adeno-associated virus capsid isolate (AAVhu69/AAVv66)
Method: EM (single particle) / Resolution: 2.46 Å

Source
  • adeno-associated virus
KeywordsVIRUS / adeno-associated virus / AAV

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