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Structure paper

TitlePelX is a UDP-N-acetylglucosamine C4-epimerase involved in Pel polysaccharide-dependent biofilm formation.
Journal, issue, pagesJ. Biol. Chem., Vol. 295, Page 11949-11962, Year 2020
Publish dateApr 13, 2020 (structure data deposition date)
AuthorsMarmont, L.S. / Whitfield, G.B. / Pfoh, R. / Williams, R.J. / Randall, T.E. / Ostaszewski, A. / Razvi, E. / Groves, R.A. / Robinson, H. / Nitz, M. ...Marmont, L.S. / Whitfield, G.B. / Pfoh, R. / Williams, R.J. / Randall, T.E. / Ostaszewski, A. / Razvi, E. / Groves, R.A. / Robinson, H. / Nitz, M. / Parsek, M.R. / Lewis, I.A. / Whitney, J.C. / Harrison, J.J. / Howell, P.L.
External linksJ. Biol. Chem. / PubMed:32601062
MethodsX-ray diffraction
Resolution2.094 - 2.11 Å
Structure data

PDB-6wj9:
UDP-GlcNAc C4-epimerase mutant S121A/Y146F from Pseudomonas protegens in complex with UDP-GlcNAc
Method: X-RAY DIFFRACTION / Resolution: 2.11 Å

PDB-6wja:
UDP-GlcNAc C4-epimerase mutant S121A/Y146F from Pseudomonas protegens in complex with UDP-GalNAc
Method: X-RAY DIFFRACTION / Resolution: 2.094 Å

PDB-6wjb:
UDP-GlcNAc C4-epimerase from Pseudomonas protegens in complex with NAD and UDP-GlcNAc
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-UD1:
URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE

ChemComp-HOH:
WATER

ChemComp-UD2:
URIDINE-DIPHOSPHATE-N-ACETYLGALACTOSAMINE

Source
  • pseudomonas protegens (bacteria)
  • pseudomonas fluorescens (strain atcc baa-477 / nrrl b-23932 / pf-5) (bacteria)
KeywordsISOMERASE / Short Chain Dehydrogenase / Complex / NAD dependent

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