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TitleCryo-EM structures of HKU2 and SADS-CoV spike glycoproteins provide insights into coronavirus evolution.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 3070, Year 2020
Publish dateJun 17, 2020
AuthorsJinfang Yu / Shuyuan Qiao / Runyu Guo / Xinquan Wang /
PubMed AbstractPorcine coronavirus SADS-CoV has been identified from suckling piglets with severe diarrhea in southern China in 2017. The SADS-CoV genome shares ~95% identity to that of bat α-coronavirus HKU2, ...Porcine coronavirus SADS-CoV has been identified from suckling piglets with severe diarrhea in southern China in 2017. The SADS-CoV genome shares ~95% identity to that of bat α-coronavirus HKU2, suggesting that SADS-CoV may have emerged from a natural reservoir in bats. Here we report the cryo-EM structures of HKU2 and SADS-CoV spike (S) glycoprotein trimers at 2.38 Å and 2.83 Å resolution, respectively. We systematically compare the domains of HKU2 spike with those of α-, β-, γ-, and δ-coronavirus spikes, showing that the S1 subunit N- and C-terminal domains of HKU2/SADS-CoV are ancestral domains in the evolution of coronavirus spike proteins. The connecting region after the fusion peptide in the S2 subunit of HKU2/SADS-CoV adopts a unique conformation. These results structurally demonstrate a close evolutionary relationship between HKU2/SADS-CoV and β-coronavirus spikes and provide insights into the evolution and cross-species transmission of coronaviruses.
External linksNat Commun / PubMed:32555182 / PubMed Central
MethodsEM (single particle)
Resolution2.38 - 2.83 Å
Structure data

30037

6m15

EMDB-30037, PDB-6m15:
Cryo-EM structures of HKU2 spike glycoproteins
Method: EM (single particle) / Resolution: 2.38 Å

30038

6m16

EMDB-30038, PDB-6m16:
Cryo-EM structures of SADS-CoV spike glycoproteins
Method: EM (single particle) / Resolution: 2.83 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-HOH:
WATER

Source
  • rhinolophus bat coronavirus hku2
  • swine acute diarrhea syndrome coronavirus
KeywordsVIRAL PROTEIN

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