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-Structure paper
タイトル | Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation. |
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ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 2663, Year 2020 |
掲載日 | 2020年5月29日 |
著者 | Aurélie Bertin / Nicola de Franceschi / Eugenio de la Mora / Sourav Maity / Maryam Alqabandi / Nolwen Miguet / Aurélie di Cicco / Wouter H Roos / Stéphanie Mangenot / Winfried Weissenhorn / Patricia Bassereau / |
PubMed 要旨 | Endosomal sorting complexes for transport-III (ESCRT-III) assemble in vivo onto membranes with negative Gaussian curvature. How membrane shape influences ESCRT-III polymerization and how ESCRT-III ...Endosomal sorting complexes for transport-III (ESCRT-III) assemble in vivo onto membranes with negative Gaussian curvature. How membrane shape influences ESCRT-III polymerization and how ESCRT-III shapes membranes is yet unclear. Human core ESCRT-III proteins, CHMP4B, CHMP2A, CHMP2B and CHMP3 are used to address this issue in vitro by combining membrane nanotube pulling experiments, cryo-electron tomography and AFM. We show that CHMP4B filaments preferentially bind to flat membranes or to tubes with positive mean curvature. Both CHMP2B and CHMP2A/CHMP3 assemble on positively curved membrane tubes. Combinations of CHMP4B/CHMP2B and CHMP4B/CHMP2A/CHMP3 are recruited to the neck of pulled membrane tubes and reshape vesicles into helical "corkscrew-like" membrane tubes. Sub-tomogram averaging reveals that the ESCRT-III filaments assemble parallel and locally perpendicular to the tube axis, highlighting the mechanical stresses imposed by ESCRT-III. Our results underline the versatile membrane remodeling activity of ESCRT-III that may be a general feature required for cellular membrane remodeling processes. |
リンク | Nat Commun / PubMed:32471988 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-10720: |
由来 |
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