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TitleArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range.
Journal, issue, pagesPlos Genet., Vol. 16, Page e1008750-e1008750, Year 2020
Publish dateNov 19, 2018 (structure data deposition date)
AuthorsGonzalez-Montes, L. / Del Campo, I. / Garcillan-Barcia, M.P. / de la Cruz, F. / Moncalian, G.
External linksPlos Genet. / PubMed:32348296
MethodsX-ray diffraction
Resolution2 - 2.7 Å
Structure data

PDB-6i89:
Crystal structure of Antirestriction ArdC protein from R388 plasmid. Metal-free structure.
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6sna:
Crystal structure of Antirestriction ArdC protein from R388 plasmid. Mn(II)-bound structure.
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

Source
  • escherichia coli (E. coli)
KeywordsMETAL BINDING PROTEIN / DNA binding protein Metalloprotease Antirestriction

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