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TitleCharting the native architecture of thylakoid membranes with single-molecule precision.
Journal, issue, pagesElife, Vol. 9, Year 2020
Publish dateApr 16, 2020
AuthorsWojciech Wietrzynski / Miroslava Schaffer / Dimitry Tegunov / Sahradha Albert / Atsuko Kanazawa / Jürgen M Plitzko / Wolfgang Baumeister / Benjamin D Engel /
PubMed AbstractThylakoid membranes scaffold an assortment of large protein complexes that work together to harness the energy of light. It has been a longstanding challenge to visualize how the intricate thylakoid ...Thylakoid membranes scaffold an assortment of large protein complexes that work together to harness the energy of light. It has been a longstanding challenge to visualize how the intricate thylakoid network organizes these protein complexes to finely tune the photosynthetic reactions. Previously, we used in situ cryo-electron tomography to reveal the native architecture of thylakoid membranes (Engel et al., 2015). Here, we leverage technical advances to resolve the individual protein complexes within these membranes. Combined with a new method to visualize membrane surface topology, we map the molecular landscapes of thylakoid membranes inside green algae cells. Our tomograms provide insights into the molecular forces that drive thylakoid stacking and reveal that photosystems I and II are strictly segregated at the borders between appressed and non-appressed membrane domains. This new approach to charting thylakoid topology lays the foundation for dissecting photosynthetic regulation at the level of single protein complexes within the cell.
External linksElife / PubMed:32297859 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-10780:
In situ cryo-electron tomogram of the Chlamydomonas chloroplast (Volta phase plate, bin4)
Method: EM (tomography)

EMDB-10781:
In situ cryo-electron tomogram of the Chlamydomonas chloroplast (Volta phase plate, bin4)
Method: EM (tomography)

EMDB-10782:
In situ cryo-electron tomogram of the Chlamydomonas chloroplast (Volta phase plate, bin4)
Method: EM (tomography)

EMDB-10783:
In situ cryo-electron tomogram of the Chlamydomonas chloroplast (Volta phase plate, bin4)
Method: EM (tomography)

Source
  • Chlamydomonas reinhardtii (plant)

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