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TitleStructure-based design approach to rational site-directed mutagenesis of beta-lactoglobulin.
Journal, issue, pagesJ. Struct. Biol., Vol. 210, Page 107493-107493, Year 2020
Publish dateJan 17, 2019 (structure data deposition date)
AuthorsBonarek, P. / Loch, J.I. / Tworzydlo, M. / Cooper, D.R. / Milto, K. / Wrobel, P. / Kurpiewska, K. / Lewinski, K.
External linksJ. Struct. Biol. / PubMed:32169624
MethodsX-ray diffraction
Resolution2 - 2.5 Å
Structure data

PDB-6qi6:
Trigonal form of WT recombinant bovine beta-lactoglobulin
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6qi7:
Engineered beta-lactoglobulin: variant L39Y in complex with endogenous ligand
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6qpd:
Engineered beta-lactoglobulin: variant I56F
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6qpe:
Engineered beta-lactoglobulin: variant L58F
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6rwp:
Engineered beta-lactoglobulin: variant L58F in complex with myristic acid
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6rwq:
Engineered beta-lactoglobulin: variant F105L in complex with myristic acid
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-6rwr:
Engineered beta-lactoglobulin: variant M107L in complex with myristic acid
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6ryt:
Engineered beta-lactoglobulin: variant M107L
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6xve:
Engineered beta-lactoglobulin: variant F105L
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

Chemicals

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-EOH:
ETHANOL

ChemComp-HOH:
WATER

ChemComp-PLM:
PALMITIC ACID

ChemComp-SO4:
SULFATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-ACT:
ACETATE ION

ChemComp-MYR:
MYRISTIC ACID

ChemComp-PO4:
PHOSPHATE ION

Source
  • bos taurus (domestic cattle)
KeywordsTRANSPORT PROTEIN / lactoglobulin / lipocalin / recombinant protein / mutant / mutation / lactoglobuin

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