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TitleStructural Analysis of Cj1427, an Essential NAD-Dependent Dehydrogenase for the Biosynthesis of the Heptose Residues in the Capsular Polysaccharides ofCampylobacter jejuni.
Journal, issue, pagesBiochemistry, Vol. 59, Page 1314-1327, Year 2020
Publish dateJan 30, 2020 (structure data deposition date)
AuthorsHuddleston, J.P. / Anderson, T.K. / Spencer, K.D. / Thoden, J.B. / Raushel, F.M. / Holden, H.M.
External linksBiochemistry / PubMed:32168450
MethodsX-ray diffraction
Resolution1.5 - 2.4 Å
Structure data

PDB-6vo6:
Crystal Structure of Cj1427, an Essential NAD-dependent Dehydrogenase from Campylobacter jejuni, in the Presence of NADH and GDP
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-6vo8:
X-ray structure of the Cj1427 in the presence of NADH and GDP-D-glycero-D-mannoheptose, an essential NAD-dependent dehydrogenase from Campylobacter jejuni
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-NAI:
1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-CL:
Unknown entry

ChemComp-TMA:
TETRAMETHYLAMMONIUM ION

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GZ0:
[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-6-oxidanylidene-3~{H}-purin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{S},4~{S},5~{S},6~{S})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl] hydrogen phosphate

Source
  • campylobacter jejuni subsp. jejuni serotype o:2 (strain atcc 700819 / nctc 11168) (Campylobacter)
KeywordsOXIDOREDUCTASE / capsular polysaccharide / isomerase / dehydrogenase

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