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TitleRevisiting the Catalytic Cycle and Kinetic Mechanism of AminoglycosideO-Nucleotidyltransferase(2′′): A Structural and Kinetic Study.
Journal, issue, pagesAcs Chem. Biol., Year 2020
Publish dateJan 8, 2015 (structure data deposition date)
AuthorsBassenden, A.V. / Park, J. / Rodionov, D. / Berghuis, A.M.
External linksAcs Chem. Biol. / PubMed:32100995
MethodsX-ray diffraction
Resolution1.82 - 1.88 Å
Structure data

PDB-4xje:
CRYSTAL STRUCTURE OF ANT(2") IN COMPLEX WITH AMP AND TOBRAMYCIN
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-5cfu:
Crystal Structure of ANT(2")-Ia in complex with adenylyl-2"-tobramycin
Method: X-RAY DIFFRACTION / Resolution: 1.82 Å

Chemicals

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-TOY:
TOBRAMYCIN / antibiotic*YM

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-MN:
Unknown entry

ChemComp-51H:
'adenylyl-2"-tobramycin'

ChemComp-POP:
PYROPHOSPHATE 2-

ChemComp-BU1:
1,4-BUTANEDIOL

Source
  • pseudomonas aeruginosa (bacteria)
Keywordstransferase/antibiotic / antibiotic resistance / nucleotidyltransferase / tobramycin / Rossmann fold / transferase-antibiotic complex / AMPCPP / modified aminoglycoside

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