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TitleA Direct Fluorescent Activity Assay for Glycosyltransferases Enables Convenient High-Throughput Screening: Application to O-GlcNAc Transferase.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 59, Page 9601-9609, Year 2020
Publish dateNov 28, 2019 (structure data deposition date)
AuthorsAlteen, M.G. / Gros, C. / Meek, R.W. / Cardoso, D.A. / Busmann, J.A. / Sangouard, G. / Deen, M.C. / Tan, H.Y. / Shen, D.L. / Russell, C.C. ...Alteen, M.G. / Gros, C. / Meek, R.W. / Cardoso, D.A. / Busmann, J.A. / Sangouard, G. / Deen, M.C. / Tan, H.Y. / Shen, D.L. / Russell, C.C. / Davies, G.J. / Robinson, P.J. / McCluskey, A. / Vocadlo, D.J.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:32092778
MethodsX-ray diffraction
Resolution1.91 Å
Structure data

PDB-6tka:
Crystal structure of human O-GlcNAc transferase bound to substrate 7 and a peptide from HCF-1 pro-repeat 2 (11-26)
Method: X-RAY DIFFRACTION / Resolution: 1.91 Å

Chemicals

ChemComp-NJ5:
[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{S},6~{R})-3-[6-(butanoylamino)hexanoylamino]-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl] hydrogen phosphate

ChemComp-NG8:
3-[2,2-bis(fluoranyl)-10,12-dimethyl-1,3-diaza-2$l^{4}-boratricyclo[7.3.0.0^{3,7}]dodeca-4,6,9,11-tetraen-4-yl]-~{N}-ethyl-propanamide

ChemComp-HOH:
WATER

Source
  • homo sapiens (human)
KeywordsTRANSFERASE / Transferase Complex O-GlcNAc OGT

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