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TitleCryo-EM structure of the 2019-nCoV spike in the prefusion conformation.
Journal, issue, pagesScience, Vol. 367, Issue 6483, Page 1260-1263, Year 2020
Publish dateMar 13, 2020
AuthorsDaniel Wrapp / Nianshuang Wang / Kizzmekia S Corbett / Jory A Goldsmith / Ching-Lin Hsieh / Olubukola Abiona / Barney S Graham / Jason S McLellan /
PubMed AbstractThe outbreak of a novel coronavirus (2019-nCoV) represents a pandemic threat that has been declared a public health emergency of international concern. The CoV spike (S) glycoprotein is a key target ...The outbreak of a novel coronavirus (2019-nCoV) represents a pandemic threat that has been declared a public health emergency of international concern. The CoV spike (S) glycoprotein is a key target for vaccines, therapeutic antibodies, and diagnostics. To facilitate medical countermeasure development, we determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the 2019-nCoV S trimer in the prefusion conformation. The predominant state of the trimer has one of the three receptor-binding domains (RBDs) rotated up in a receptor-accessible conformation. We also provide biophysical and structural evidence that the 2019-nCoV S protein binds angiotensin-converting enzyme 2 (ACE2) with higher affinity than does severe acute respiratory syndrome (SARS)-CoV S. Additionally, we tested several published SARS-CoV RBD-specific monoclonal antibodies and found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs. The structure of 2019-nCoV S should enable the rapid development and evaluation of medical countermeasures to address the ongoing public health crisis.
External linksScience / PubMed:32075877 / PubMed Central
MethodsEM (single particle)
Resolution3.17 - 3.46 Å
Structure data

EMDB-21374:
2019-nCoV spike glycoprotein with C3 symmetry imposed
Method: EM (single particle) / Resolution: 3.17 Å

21375

6vsb

EMDB-21375, PDB-6vsb:
Prefusion 2019-nCoV spike glycoprotein with a single receptor-binding domain up
Method: EM (single particle) / Resolution: 3.46 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • severe acute respiratory syndrome coronavirus 2
KeywordsVIRAL PROTEIN / Spike / Fusion glycoprotein / Coronavirus

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