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Structure paper

TitleExpanding crystallization tools for nucleic acid complexes using U1A protein variants.
Journal, issue, pagesJ. Struct. Biol., Vol. 210, Page 107480-107480, Year 2020
Publish dateSep 4, 2019 (structure data deposition date)
AuthorsRosenbach, H. / Victor, J. / Borggrafe, J. / Biehl, R. / Steger, G. / Etzkorn, M. / Span, I.
External linksJ. Struct. Biol. / PubMed:32070773
MethodsX-ray diffraction
Resolution1.84 - 2.45 Å
Structure data

PDB-6sqn:
Structure of the U1A variant A1-98 Y31H/Q36R/F56W triple mutant co-crystallized with RNA
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-6sqq:
Structure of the U1A variant A1-98 Y31H/Q36R/F56W triple mutant in complex with RNA obtained by soaking
Method: X-RAY DIFFRACTION / Resolution: 2.37 Å

PDB-6sqt:
Structure of the U1A variant A1-98 Y31H/Q36R/F56W triple mutant
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-6sqv:
Structure of the U1A variant A1-98 Y31H/Q36R/R70W
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-6sr7:
Structure of the U1A variant A1-98 Y31H/Q36R/K98W
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-SO4:
SULFATE ION

Source
  • homo sapiens (human)
KeywordsRNA BINDING PROTEIN / U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A / U1A / RNA hairpin / co-crystallization / soaking

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