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TitlePhotoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy.
Journal, issue, pagesNat Commun, Vol. 11, Page 741-741, Year 2020
Publish dateOct 10, 2019 (structure data deposition date)
AuthorsWoodhouse, J. / Nass Kovacs, G. / Coquelle, N. / Uriarte, L.M. / Adam, V. / Barends, T.R.M. / Byrdin, M. / de la Mora, E. / Bruce Doak, R. / Feliks, M. ...Woodhouse, J. / Nass Kovacs, G. / Coquelle, N. / Uriarte, L.M. / Adam, V. / Barends, T.R.M. / Byrdin, M. / de la Mora, E. / Bruce Doak, R. / Feliks, M. / Field, M. / Fieschi, F. / Guillon, V. / Jakobs, S. / Joti, Y. / Macheboeuf, P. / Motomura, K. / Nass, K. / Owada, S. / Roome, C.M. / Ruckebusch, C. / Schiro, G. / Shoeman, R.L. / Thepaut, M. / Togashi, T. / Tono, K. / Yabashi, M. / Cammarata, M. / Foucar, L. / Bourgeois, D. / Sliwa, M. / Colletier, J.P. / Schlichting, I. / Weik, M.
External linksNat Commun / PubMed:32029745
MethodsX-ray diffraction
Resolution1.6 - 1.85 Å
Structure data

PDB-6t39:
Crystal structure of rsEGFP2 in its off-state determined by SFX
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6t3a:
Difference-refined structure of rsEGFP2 10 ns following 400-nm laser irradiation of the off-state determined by SFX
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / rsEGFP2 / Green Fluorescent Protein / RSFP

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