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TitleStructural insights into ATP hydrolysis by the MoxR ATPase RavA and the LdcI-RavA cage-like complex.
Journal, issue, pagesCommun Biol, Vol. 3, Issue 1, Page 46, Year 2020
Publish dateJan 28, 2020
AuthorsMatthew Jessop / Benoit Arragain / Roger Miras / Angélique Fraudeau / Karine Huard / Maria Bacia-Verloop / Patrice Catty / Jan Felix / Hélène Malet / Irina Gutsche /
PubMed AbstractThe hexameric MoxR AAA+ ATPase RavA and the decameric lysine decarboxylase LdcI form a 3.3 MDa cage, proposed to assist assembly of specific respiratory complexes in E. coli. Here, we show that ...The hexameric MoxR AAA+ ATPase RavA and the decameric lysine decarboxylase LdcI form a 3.3 MDa cage, proposed to assist assembly of specific respiratory complexes in E. coli. Here, we show that inside the LdcI-RavA cage, RavA hexamers adopt an asymmetric spiral conformation in which the nucleotide-free seam is constrained to two opposite orientations. Cryo-EM reconstructions of free RavA reveal two co-existing structural states: an asymmetric spiral, and a flat C2-symmetric closed ring characterised by two nucleotide-free seams. The closed ring RavA state bears close structural similarity to the pseudo two-fold symmetric crystal structure of the AAA+ unfoldase ClpX, suggesting a common ATPase mechanism. Based on these structures, and in light of the current knowledge regarding AAA+ ATPases, we propose different scenarios for the ATP hydrolysis cycle of free RavA and the LdcI-RavA cage-like complex, and extend the comparison to other AAA+ ATPases of clade 7.
External linksPubMed:31992852 / Publisher's page
KeywordsHYDROLASE / Complex / MoxR ATPase / Lysine decarboxylase / CHAPERONE / AAA+ ATPase / MoxR / Escherichia coli
MethodsEM (single particle)
Resolution6 - 7.8 A
Structure data

EMDB-4469:
Spiral structure of E. coli RavA in the RavA-LdcI cage-like complex

EMDB-4470:
Spiral structure of E. coli RavA in the RavA-LdcI cage-like complex

PDB-6q7l:
Spiral structure of E. coli RavA in the RavA-LdcI cage-like complex

PDB-6q7m:
Spiral structure of E. coli RavA in the RavA-LdcI cage-like complex

EMDB-10351:
MoxR AAA-ATPase RavA, C2-symmetric closed ring conformation

EMDB-10352:
MoxR AAA-ATPase RavA, spiral open ring conformation

PDB-6sza:
MoxR AAA-ATPase RavA, C2-symmetric closed ring conformation

PDB-6szb:
MoxR AAA-ATPase RavA, spiral open ring conformation

Chemicals

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE / Pyridoxal phosphate

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • escherichia coli (E. coli)
  • escherichia coli k-12 (bacteria)

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