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Structure paper

TitleStructure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance.
Journal, issue, pagesBiotechnol Biofuels, Vol. 13, Page 5-5, Year 2020
Publish dateOct 24, 2019 (structure data deposition date)
AuthorsLee, M. / Rozeboom, H.J. / Keuning, E. / de Waal, P. / Janssen, D.B.
External linksBiotechnol Biofuels / PubMed:31938040
MethodsX-ray diffraction
Resolution1.86 - 2 Å
Structure data

PDB-6t8e:
Crystal structure of native xylose isomerase from Piromyces E2 grown in yeast, in complex with xylose
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

PDB-6t8f:
Crystal structure of mutant xylose isomerase (V270A/A273G) from Piromyces E2 grown in yeast, in complex with xylose
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-XLS:
D-xylose

ChemComp-XYP:
beta-D-xylopyranose

ChemComp-XYS:
alpha-D-xylopyranose

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • piromyces sp. (strain e2) (fungus)
KeywordsISOMERASE / TIM-barrel

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