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Structure paper

TitleMolecular and structural characterization of oxidized ribonucleotide insertion into DNA by human DNA polymerase beta.
Journal, issue, pagesJ. Biol. Chem., Vol. 295, Page 1613-1622, Year 2020
Publish dateOct 15, 2019 (structure data deposition date)
AuthorsSmith, M.R. / Alnajjar, K.S. / Hoitsma, N.M. / Sweasy, J.B. / Freudenthal, B.D.
External linksJ. Biol. Chem. / PubMed:31892517
MethodsX-ray diffraction
Resolution1.936 - 2.55 Å
Structure data

PDB-6uok:
Y271G DNA polymerase beta substrate complex with templating cytosine and incoming r8-oxo-GTP
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-6uol:
Y271G DNA polymerase beta substrate complex with a templating cytosine and incoming rGTP
Method: X-RAY DIFFRACTION / Resolution: 1.936 Å

PDB-6uom:
Y271G DNA polymerase beta ternary complex with templating adenine and incoming r8-oxo-GTP
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-MN:
Unknown entry

ChemComp-8GT:
8-OXO-GUANOSINE-5'-TRIPHOSPHATE

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-DOC:
2',3'-DIDEOXYCYTIDINE-5'-MONOPHOSPHATE

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsTRANSFERASE/DNA / DNA Polymerase / oxidized ribonucleotide / DNA damage / DNA BINDING PROTEIN / TRANSFERASE-DNA complex / ribonucleotide / oxidized nucleotide

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