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Structure paper

TitleAttenuated substrate inhibition of a haloketone reductase via structure-guided loop engineering.
Journal, issue, pagesJ. Biotechnol., Vol. 308, Page 141-147, Year 2020
Publish dateNov 28, 2017 (structure data deposition date)
AuthorsShang, Y.P. / Chen, Q. / Li, A.T. / Quan, S. / Xu, J.H. / Yu, H.L.
External linksJ. Biotechnol. / PubMed:31866427
MethodsX-ray diffraction
Resolution2.039 - 2.098 Å
Structure data

PDB-5yw4:
Structure-Guided Engineering of Reductase: Efficient Attenuating Substrate Inhibition in Asymmetric Catalysis
Method: X-RAY DIFFRACTION / Resolution: 2.047 Å

PDB-5ywl:
SsCR_L211H
Method: X-RAY DIFFRACTION / Resolution: 2.098 Å

PDB-5ywn:
SsCR_L211H-NADP+
Method: X-RAY DIFFRACTION / Resolution: 2.039 Å

Chemicals

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-HOH:
WATER

Source
  • scheffersomyces stipitis (fungus)
  • scheffersomyces stipitis cbs 6054 (fungus)
  • scheffersomyces stipitis (strain atcc 58785 / cbs 6054 / nbrc 10063 / nrrl y-11545) (fungus)
KeywordsOXIDOREDUCTASE / reductase / NADP+ / asymmetric catalysis / substrate inhibition / asymmetric reduction

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