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Structure paper

TitleCrucial Roles of Two Hydrated Mg2+Ions in Reaction Catalysis of the Pistol Ribozyme.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 59, Page 2837-2843, Year 2020
Publish dateSep 23, 2019 (structure data deposition date)
AuthorsTeplova, M. / Falschlunger, C. / Krasheninina, O. / Egger, M. / Ren, A. / Patel, D.J. / Micura, R.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:31804735
MethodsX-ray diffraction
Resolution2.645 - 3.2 Å
Structure data

PDB-6uey:
Pistol ribozyme transition-state analog vanadate
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6uf1:
Pistol ribozyme transition-state analog vanadate
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-6ufj:
Pistol ribozyme product crystal structure
Method: X-RAY DIFFRACTION / Resolution: 2.645 Å

PDB-6ufk:
Pistol ribozyme product crystal soaked in Mn2+
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-MN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsRNA / Self-cleaving ribozymes / transesterification reaction / pre-catalytic state / pentavalent transition state analog / cyclophosphate product

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