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Structure paper

TitleDiscovery of a Cryptic Intermediate in Late Steps of Mithramycin Biosynthesis.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 59, Page 826-832, Year 2020
Publish dateMay 8, 2019 (structure data deposition date)
AuthorsWheeler, R. / Yu, X. / Hou, C. / Mitra, P. / Chen, J.M. / Herkules, F. / Ivanov, D.N. / Tsodikov, O.V. / Rohr, J.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:31702856
MethodsX-ray diffraction
Resolution1.8 - 2.67 Å
Structure data

PDB-6ovq:
Crystal structure of mithramycin 3-side chain keto-reductase MtmW
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6ovx:
Crystal structure of mithramycin 3-side chain keto-reductase MtmW in complex with NAD+, P422 form
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6ow0:
Crystal structure of mithramycin 3-side chain keto-reductase MtmW in complex with NAD+ and PEG
Method: X-RAY DIFFRACTION / Resolution: 2.67 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • streptomyces argillaceus (bacteria)
KeywordsOXIDOREDUCTASE / Natural product / Biosynthesis / Aureolic acid / Reductase

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