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TitleEngineered C-N Lyase: Enantioselective Synthesis of Chiral Synthons for Artificial Dipeptide Sweeteners.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 59, Page 429-435, Year 2020
Publish dateJun 7, 2019 (structure data deposition date)
AuthorsZhang, J. / Grandi, E. / Fu, H. / Saravanan, T. / Bothof, L. / Tepper, P.G. / Thunnissen, A.W.H. / Poelarends, G.J.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:31625664
MethodsX-ray diffraction
Resolution1.44 - 1.92 Å
Structure data

PDB-6rx8:
EDDS lyase variant D290M/Y320M with bound fumarate
Method: X-RAY DIFFRACTION / Resolution: 1.92 Å

PDB-6rxa:
EDDS lyase variant D290M/Y320M with bound formate
Method: X-RAY DIFFRACTION / Resolution: 1.44 Å

Chemicals

ChemComp-FUM:
FUMARIC ACID

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-FMT:
FORMIC ACID

ChemComp-GOL:
GLYCEROL

Source
  • chelativorans sp. (strain bnc1) (bacteria)
KeywordsLYASE / EDDS lyase / C-N lyase / aspartase/fumarase / protein engineering

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