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Structure paper

TitleMolecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease.
Journal, issue, pagesPlos Biol., Vol. 17, Page e3000496-e3000496, Year 2019
Publish dateMay 22, 2019 (structure data deposition date)
AuthorsChen, W. / Zhang, H. / Zhang, Y. / Wang, Y. / Gan, J. / Ji, Q.
External linksPlos Biol. / PubMed:31603896
MethodsX-ray diffraction
Resolution2.7 - 3.2 Å
Structure data

PDB-6k3z:
Crystal structure of dCas9 in complex with sgRNA and DNA (TGA PAM)
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-6k4p:
Crystal structure of xCas9 in complex with sgRNA and DNA (TGG PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-6k4q:
Crystal structure of xCas9 in complex with sgRNA and DNA (CGG PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-6k4s:
Crystal structure of xCas9 in complex with sgRNA and DNA (TGC PAM)
Method: X-RAY DIFFRACTION / Resolution: 3.01 Å

PDB-6k4u:
Crystal structure of xCas9 in complex with sgRNA and DNA (TGA PAM)
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-6k57:
Crystal structure of dCas9 in complex with sgRNA and DNA (CGA PAM)
Method: X-RAY DIFFRACTION / Resolution: 2.98 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

Source
  • streptococcus pyogenes serotype m1 (bacteria)
  • streptococcus pyogenes (bacteria)
KeywordsHYDROLASE/DNA/RNA / endonuclease / HYDROLASE-DNA-RNA complex / HYDROLASE/DNA / HYDROLASE-DNA complex / HYDROLASE/RNA/DNA / HYDROLASE-RNA-DNA complex

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