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TitleMutagenesis and Structural Studies Reveal the Basis for the Activity and Stability Properties That Distinguish thePhotinusLuciferasesscintillansandpyralis.
Journal, issue, pagesBiochemistry, Vol. 58, Page 4293-4303, Year 2019
Publish dateAug 8, 2019 (structure data deposition date)
AuthorsBranchini, B.R. / Fontaine, D.M. / Southworth, T.L. / Huta, B.P. / Racela, A. / Patel, K.D. / Gulick, A.M.
External linksBiochemistry / PubMed:31560532
MethodsX-ray diffraction
Resolution2.75 Å
Structure data

PDB-6q2m:
Crystal structure of Photinus pyralis Luciferase Pps6 mutant in complex with DLSA
Method: X-RAY DIFFRACTION / Resolution: 2.75 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-DYD:
(2S,5S)-hexane-2,5-diol

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-SLU:
5'-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL] ADENOSINE

ChemComp-HOH:
WATER

Source
  • photinus pyralis (common eastern firefly)
KeywordsLIGASE / Luciferase / adenylation domain / Bioluminescence

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