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Structure paper

TitleProtein crystal structure determination with the crystallophore, a nucleating and phasing agent.
Journal, issue, pagesJ. Appl. Crystallogr., Vol. 52, Page 722-731, Year 2019
Publish dateAug 21, 2018 (structure data deposition date)
AuthorsEngilberge, S. / Wagner, T. / Santoni, G. / Breyton, C. / Shima, S. / Franzetti, B. / Riobe, F. / Maury, O. / Girard, E.
External linksJ. Appl. Crystallogr. / PubMed:31396026
MethodsX-ray diffraction
Resolution1.96 - 2.55 Å
Structure data

PDB-6hf6:
Crystal structure of the Protease 1 (E29A,E60A,E80A) from Pyrococcus horikoshii co-crystallized with Tb-Xo4.
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6hf7:
Crystal structure of the adenylate kinase from Methanothermococcus thermolithotrophicus co-crystallized with Tb-Xo4
Method: X-RAY DIFFRACTION / Resolution: 1.96 Å

PDB-6hk1:
Crystal structure of the Thiazole synthase from Methanothermococcus thermolithotrophicus co-crystallized with Tb-Xo4
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

Chemicals

ChemComp-7MT:
Tb-Xo4

ChemComp-MLI:
MALONATE ION

ChemComp-TB:
TERBIUM(III) ION

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-48F:
[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3R)-2,3,5-tris(oxidanyl)-4-oxidanylidene-pentyl] hydrogen phosphate

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-NA:
Unknown entry

Source
  • pyrococcus horikoshii (strain atcc 700860 / dsm 12428 / jcm 9974 / nbrc 100139 / ot-3) (archaea)
  • methanothermococcus thermolithotrophicus (archaea)
KeywordsHYDROLASE / meshandcollect / de novo phasing / SAD / crystallization / Tb-Xo4 / crystallophore / Lanthanide complex / multi-crystals data collection / ccCluster / TRANSFERASE / Protein crystallization / nucleation / molecular glue / BIOSYNTHETIC PROTEIN / native purification / nucleating agent

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