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Structure paper

TitleNucleotide signaling pathway convergence in a cAMP-sensing bacterial c-di-GMP phosphodiesterase.
Journal, issue, pagesEmbo J., Vol. 38, Page e100772-e100772, Year 2019
Publish dateSep 24, 2018 (structure data deposition date)
AuthorsCadby, I.T. / Basford, S.M. / Nottingham, R. / Meek, R. / Lowry, R. / Lambert, C. / Tridgett, M. / Till, R. / Ahmad, R. / Fung, R. ...Cadby, I.T. / Basford, S.M. / Nottingham, R. / Meek, R. / Lowry, R. / Lambert, C. / Tridgett, M. / Till, R. / Ahmad, R. / Fung, R. / Hobley, L. / Hughes, W.S. / Moynihan, P.J. / Sockett, R.E. / Lovering, A.L.
External linksEmbo J. / PubMed:31355487
MethodsX-ray diffraction
Resolution2.45 - 2.83 Å
Structure data

PDB-6hq2:
Structure of EAL Enzyme Bd1971 - apo form
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-6hq3:
Structure of EAL Enzyme Bd1971 - halfsite-occupied form
Method: X-RAY DIFFRACTION / Resolution: 2.79 Å

PDB-6hq4:
Structure of EAL enzyme Bd1971 - cAMP bound form
Method: X-RAY DIFFRACTION / Resolution: 2.63 Å

PDB-6hq5:
Structure of EAL Enzyme Bd1971 - cAMP and cyclic-di-GMP bound form
Method: X-RAY DIFFRACTION / Resolution: 2.83 Å

PDB-6hq7:
Structure of EAL Enzyme Bd1971 - cGMP bound form
Method: X-RAY DIFFRACTION / Resolution: 2.46 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-CMP:
ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

ChemComp-MG:
Unknown entry

ChemComp-C2E:
9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)

ChemComp-CA:
Unknown entry

ChemComp-PCG:
CYCLIC GUANOSINE MONOPHOSPHATE

Source
  • bdellovibrio bacteriovorus (strain atcc 15356 / dsm 50701 / ncib 9529 / hd100) (bacteria)
KeywordsSIGNALING PROTEIN / EAL / cyclic-di-GMP / cAMP / Bdellovibrio

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